Journal: Cancers

Article Title: Protein Disulphide Isomerase A1 Is Involved in the Regulation of Breast Cancer Cell Adhesion and Transmigration via Lung Microvascular Endothelial Cells

doi: 10.3390/cancers12102850

Figure Lengend Snippet: Effects of PDIA1 inhibition on wound-healing and migration of breast cancer cells and endothelial cells. ECIS Wound-Healing Assay of hLMVEC, MCF-7 and MDA-MB-231 cells and breast cancer sublines with silencing of PDIA1 (shN, shPDIA1-1 and shPDIA1-3). Real time tracings in hLMVECs ( A ), MCF-7 ( C ) and MDA-MB-231 ( E ) cell lines after addition of bepristat 2a at various concentrations (1, 10, 30 or 50 µM) and after PDIA1 silencing in MCF-7 ( G ) and MDA-MB-231 ( I ) cell lines. Area under the curve boxplots represent AUC quantitation of changes in migration rate of bepristat 2a-treated hLMVECs ( B ), MCF-7 ( D ) and MDA-MB-231 ( F ) cell lines versus non-treated controls as well as MCF-7 ( H ) or MDA-MB-231 ( J ) sublines transduced against PDIA1 (shPDIA1-1, shPDIA1-3) or wild type cells regarding to negative sequence (shN). The line graphs and AUC boxplots represent mean ± SD of three independent experiments. Statistical analysis was calculated using parametric one-way ANOVA followed by Dunnett’s multiple comparisons test (* p = 0.05, ** p = 0.01, *** p = 0.001).

Article Snippet: Human lung microvascular endothelial cell line (hLMVECs) was obtained from Cell Applications (San Diego, CA, USA).

Techniques: Inhibition, Migration, Wound Healing Assay, Quantitation Assay, Sequencing

Journal: Cancers

Article Title: Protein Disulphide Isomerase A1 Is Involved in the Regulation of Breast Cancer Cell Adhesion and Transmigration via Lung Microvascular Endothelial Cells

doi: 10.3390/cancers12102850

Figure Lengend Snippet: Effects of exogenous PDIA1 and PDIA3 on adhesive interaction between breast cancer cells and different substrates. Effect of exogenous proteins PDIA1 and PDIA3 on adhesion of MCF-7 ( A – F ) and MDA-MB-231 ( G – L ) cells to collagen type I, fibronectin and lung microvascular hLMVEC cells, respectively. Data represent mean ± SD of three independent experiments. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s multiple comparisons test (* p = 0.05, ** p = 0.01, *** p = 0.001).

Article Snippet: Human lung microvascular endothelial cell line (hLMVECs) was obtained from Cell Applications (San Diego, CA, USA).

Techniques: Adhesive

Journal: Oncology Letters

Article Title: MicroRNA-1294 inhibited oral squamous cell carcinoma growth by targeting c-Myc

doi: 10.3892/ol.2018.8967

Figure Lengend Snippet: Relatively low level of miR-1294 in OSCC tissue samples. (A) The representative image of OSCC tissues HE staining (magnification, ×200). The miR-1294 levels of 24 OSCC tissues samples and their matched adjacent normal tissues were assessed by qRT-PCR. Relative miR-1294 levels tumor/normal (log2) were listed (B); the mean miR-1294 values of the OSCC tissues and their matched adjacent normal tissues were also recorded. (C) *P<0.05 vs. Normal tissues. (D) The miR-545 levels of 5 OSCC cell lines (HSC2, HSC4, SAS and KON) and primary gingival keratinocytes (PGK) were assayed by qRT-PCR. *P<0.05 vs. PGK. These experiments were performed in triplicate. OSCC, oral squamous cell carcinoma.

Article Snippet: Primary gingival keratinocytes (PGK; ATCC ® PCS-200-014TM) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA) and cultured in Dermal Cell Basal Medium (ATCC ® PCS-200-030TM) plus one Keratinocyte Growth Kit (ATCC ® PCS-200-040TM).

Techniques: Staining, Quantitative RT-PCR